LOCUS       40924_49127        5124 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD023, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 5124)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5124)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Division of Biological Sciences, University of 
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    46..51
                     /label=ZraI
                     /note="ZraI"
     misc_feature    49..69
                     /note="G5C5; GC-adaptor for seamless assembly, flanking a
                     BHR replicons, neutral sites, or cyanobacterial replicons; 
                     synthetic"
     misc_feature    70..75
                     /label=XbaI
                     /note="XbaI"
     misc_recomb     76..536
                     /note="S7942NS2-RA; right arm of the homologous
                     recombination site NS2 into the chromosome of Synechococcus
                     elongatus PCC7942 derived from INSD accession NC_007604, 
                     U44761"
     misc_feature    445..450
                     /label=SacI
                     /note="SacI"
     rep_origin      573..1161
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(1347..1487)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     misc_feature    complement(1567..1656)
                     /note="tet-3UTR; 3' UTR of the tetracyclin resistance ORF;
                     from pACYC184 INSD accession X06403"
     CDS             complement(1660..2847)
                     /label=TcR
                     /note="tetracycline efflux protein"
     promoter        complement(2895..2923)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     misc_recomb     2972..3913
                     /note="S7942NS2-LA; left arm of the homologous
                     recombination site NS2 into the chromosome of Synechococcus
                     elongatus PCC7942 derived from INSD accession NC_007604, 
                     U44761"
     misc_feature    3914..3919
                     /label=NheI
                     /note="NheI"
     misc_feature    3920..3940
                     /note="GC; GC-adaptor for seamless assembly, flanking BHR 
                     replicons or neutral sites; synthetic"
     misc_feature    3938..3943
                     /label=ZraI
                     /note="ZraI"
     CDS             complement(4067..4924)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4925..5029)
                     /label=AmpR promoter
     misc_feature    5055..5060
                     /label=ZraI
                     /note="ZraI"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagcgacgt cgggggcccc
       61 cggggggatt ctagagttgc taccaagtag tgacccgctt cgtgatgcaa aatccgctga
      121 cgatattcgg gcgatcgctg ctgaatgcca tcgagcagta acgtggcacc ccgcccctgc
      181 caagtcaccg catccagact gaacagcacc aagaggctaa aacccaatcc cgccggtagc
      241 agcggagaac tacccagcat tggtcccacc aaagctaatg ccgtcgtggt aaaaatcgcg
      301 atcgccgtca gactcaagcc cagttcgctc atgcttcctc atctaggtca cagtcttcgg
      361 cgatcgcatc gatctgatgc tgcagcaagc gttttccata ccggcgatcg cgccgtcgcc
      421 ctttcgctgc cgtggcccgc ttacgagctc gtttatcgac cacgatcgca tccaaatccg
      481 cgatcgcttc ccagtccggc aattcagtct ggggcgtccg tttcattaat cctgatagcg
      541 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
      601 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
      661 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
      721 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
      781 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
      841 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
      901 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
      961 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     1021 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     1081 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     1141 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     1201 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     1261 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     1321 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
     1381 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     1441 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
     1501 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     1561 ctccgctggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag
     1621 tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca
     1681 tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc
     1741 caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccaat
     1801 gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc
     1861 atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag
     1921 aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc
     1981 cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc
     2041 gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag
     2101 gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc
     2161 cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt
     2221 catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg
     2281 acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt
     2341 gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc
     2401 cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc
     2461 ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc
     2521 ggtgatgccg gccacgatgc gtccggcgta gaggatccac aggacgggtg tggtcgccat
     2581 gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa
     2641 gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc
     2701 tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc
     2761 cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat
     2821 gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg
     2881 tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattacaact
     2941 tatatcgtat ggggctgact tcaggtggcg ggccacgatt tgagggacga atcttcagcg
     3001 tgactgaagt acctcgcggc aagccatttc ccgatgtctt tttgttggcc gccgatcgct
     3061 tcggggttaa tcctacggcc tgcgctgtga tcgaagacac ccccttggga gtagcggcag
     3121 gcgtggcggc aggaatgcaa gtgtttggct acgcgggttc catgcccgct tggcgtctgc
     3181 aagaagccgg tgcccatctc atttttgacg atatgcgact gctgcccagt ctgctccaat
     3241 cgtcgccaaa agataactcc acagcattgc ccaatcccta acccctgctc gcgccgcaac
     3301 tacacactaa accgttcctg cgcgatcgct cttactgttg atggctcgtg cttaaaaaca
     3361 atgcaaccct aaccgtttca gctggtgatt ttcggacgat ttggcttaca gggataactg
     3421 agagtcaaca gcctctgtcc gtcattgcac acccatccat gcactgggga cttgactcat
     3481 gctgaatcac atttcccttg tccattgggc gagaggggag gggaatcttc tggactcttc
     3541 actaagcggc gatcgcaggt tcttctaccc aagcagtggc gatcgcttga ttgcagtctt
     3601 caatgctggc ctctgcagcc atcgccgcca ccaaagcatc gtaggcggga cgttgttgct
     3661 ccagtaaagt cttcgcccgt aacaatcccc agcgactgcg taaatccgct tcggcaggat
     3721 tgcgatcgag ttgccgccac agttgtttcc actgggcgcg atcgtcagct cccccttcca
     3781 cgttgccgta gaccagttgc tctgccgctg caccggccat caacacctga caccactgtt
     3841 ccagcgatcg ctgactgagt tgcccctgtg cggcttcggc ttctagcgca gctgcttgga
     3901 actgcacacc cccgctagca tcgcgcgcgc gcgcgcggac gtcctcacgt taagggattt
     3961 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     4021 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     4081 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     4141 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     4201 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     4261 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     4321 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     4381 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     4441 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     4501 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     4561 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4621 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     4681 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     4741 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     4801 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     4861 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     4921 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     4981 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     5041 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     5101 ggcgtatcac gaggcccttt cgtc
//
